how is southern blotting done ?

Description

https://en.wikipedia.org/wiki/Southern_blot

https://en.wikipedia.org/wiki/Southern_blot
Southern blot – Wikipedia
The Southern blotting combines the transfer of electrophoresis -separated DNA fragments to a filter membrane in a process called blotting, and the subsequent fragment detection by probe hybridization. [2] The method is named after the British biologist Edwin Southern, who first published it in 1975. [3]

https://askabiologist.asu.edu/southern-blotting

https://askabiologist.asu.edu/southern-blotting
Southern Blotting Technique | Ask A Biologist
1. Digest the DNA with an appropriate restriction enzyme. 2. Run the digest on an agarose gel. 3. Denature the DNA (usually while it is still on the gel). For example, soak it in about 0.5M NaOH, which would separate double-stranded DNA into single-stranded DNA. Only ssDNA can transfer. A depurination step is optional.
Thunderbird School of Global ManagementPCRPlasmidsAlkaline LysisElectrophoresisContact ASU

https://microbeonline.com / southern-blotting-principle-steps-and-applications

https://microbeonline.com / southern-blotting-principle-steps-and-applications
Southern Blotting: Principle, Steps, Applications – Microbe Online
29 déc. 2023Southern blotting is a molecular technique to find target DNA sequences in a sample. It is a multi-step process, that begins with the electrophoresis of DNA, transfer of DNA fragments into nitrocellulose strip, and exposing those fragments to a DNA probe labeled with a radioactive or chemical tag.

https://www.genome.gov/genetics-glossary/Southern-Blot

https://www.genome.gov/genetics-glossary/Southern-Blot
Southern Blot – National Human Genome Research Institute
Il y a 2 jours… Southern blot. In human tumors, changes in the number of copies or the structure of cancer-causing genes are frequent. Southern blot analysis can be used to investigate whether a gene is amplified, deleted, or structurally rearranged in cancer cells as compared to normal cells.

https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/gel-electrophoresis/southern-and-northern-blotting

https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/gel-electrophoresis/southern-and-northern-blotting
Northern and Southern Blot Protocols & Introduction – MilliporeSigma
Southern and Northern blotting protocols involve the following major steps: Purification of DNA/RNA: Extract and purify the DNA/RNA from either cells or tissue sources. Digestion of DNA: Digest the DNA into fragments with restriction enzymes. This step is not required for RNA. Gel electrophoresis: Separate the DNA fragments on agarose gel.

https://www.khanacademy.org/test-prep/mcat/biomolecules/dna-technology/v/southern-blot

https://www.khanacademy.org/test-prep/mcat/biomolecules/dna-technology/v/southern-blot
Southern blot (video) | DNA technology | Khan Academy
Southern Blotting is a technique used to identify specific DNA sequences in a complex mixture. It involves cutting DNA into fragments, separating them by size through gel electrophoresis, transferring them onto a filter, and exposing them to a radio-labeled DNA probe. This process helps visualize the presence of a gene of interest.

https://cshprotocols.cshlp.org/content/2021/7/pdb.prot100487.full

https://cshprotocols.cshlp.org/content/2021/7/pdb.prot100487.full
Southern Blotting – CSH Protocols
When the blotting paper on the top of the support is thoroughly wet, smooth out all air bubbles with a glass rod or pipette. 12. Remove the gel from the solution in Step 7, and invert it so that its underside is now uppermost. Place the inverted gel on the support so that it is centered on the wet blotting paper.

https://www.nature.com/wls/definition/southern-blot-289

https://www.nature.com/wls/definition/southern-blot-289
Southern blot | World Library of Science – Nature
Southern blot. A Southern blot is a laboratory method used to detect specific DNA molecules from among a many other DNA molecules. The technique was named after its inventor, Edward Southern. As a …

https://bio.libretexts.org/Bookshelves/Genetics/Online_Open_Genetics_(Nickle_and_Barrette-Ng)/08:_Techniques_of_Molecular_Genetics/8.07:__DNA_Analysis-_Blotting_and_Hybridization

https://bio.libretexts.org/Bookshelves/Genetics/Online_Open_Genetics_(Nickle_and_Barrette-Ng)/08:_Techniques_of_Molecular_Genetics/8.07:__DNA_Analysis-_Blotting_and_Hybridization
8.7: DNA Analysis- Blotting and Hybridization – Biology LibreTexts
20 juin 2023Figure 8.7.3 8.7. 3: Comparison of Southern, Northern, and Western blots. Size and amount of DNA, RNA, and polypeptides can be determined using similar blotting methods. DNA is in blue, RNA in red, and polypeptides in green. A marker lane is shown in the left of each gel to determine size. A eukaryote cell is shown, but the same methods can be …

https://experiments.springernature.com/articles/10.1385/0-89603-205-1:147

https://experiments.springernature.com/articles/10.1385/0-89603-205-1:147
The Southern Blot | Springer Nature Experiments
The Southern blot technique, published by Southern in 1975 (1), is probably the most widely used method in molecular biology and, to date, has received in excess of 100,000 literature citations. The purpose of the method is the detection of gel-fractionated DNA molecules following transfer to a membrane. lesoutrali bot